Table 5.
Peptide ion validation data from CID MS/MS mass spectrometry which corresponds with Table 4.
| Spot no. | Protein name | Accession number | Verification CID ions | Peptide position in protein sequence: start–end | m/z | Ions score | Amino acid sequence | Variable modification: carbymidomethyl cysteine (C), oxidation methionine (M) |
|---|---|---|---|---|---|---|---|---|
| 20 | Albumin, isoform CRA-t | 119626083 | 2 | 162–168 | 927.5 | 21 | K.YLYEIAR.R | |
| 398–405 | 960.57 | 23 | K.FQNALLVR.Y | |||||
| 26 | Leucine -rich alpha-2-glycoprotein 1 | 16418467 | 2 | 165 –175 | 1152.62 | 79 | K.ALGHLDLSGNR.L | |
| 251–260 | 989.56 | 83 | R.VAAGAFQGLR.Q | |||||
| 27 | HP protein | 47124562 | 3 | 153–161 | 980.51 | 7 | R.VGYVSGWGR.N | |
| 173–186 | 1707.82 | 30 | K.YVMLPVADQDQCIR.H | C | ||||
| 173–186 | 1723.81 | 41 | K.YVMLPVADQDQCIR.H | C, M | ||||
| 32 | Albumin, isoform CRA-k | 119626074 | 3 | 169–180 | 1467.87 | 16 | R.RHPDYSVVLLLR.L | |
| 170–180 | 1311.76 | 12 | R.HPDYSVVLLLR.L | |||||
| 235–242 | 960.59 | 56 | K.FQNALLVR.Y | |||||
| 37 | Apolipoprotein A-II isoform CRA_c | 119573006 | 1 | 48–57 | 1156.69 | 75 | K.SKEQLTPLIK.K | |
| 39 | No identification | – | – | |||||
| 41 | Serum Albumin with S-Naproxen, Chain A | 168988718 | 8 | 60–68 | 1017.55 | 39 | K.SLHTLFGDK.L | |
| 89–101 | 1714.83 | 27 | K.QEPERNECFLQHK.D | C | ||||
| 133–139 | 927.52 | 36 | K.YLYEIAR.R | |||||
| 332–343 | 1467.87 | 26 | R.RHPDYSVVLLLR.L | |||||
| 333–343 | 1311.77 | 19 | R.HPDYSVVLLLR.L | |||||
| 398–405 | 960.59 | 41 | K.FQNALLVR.Y | |||||
| 409–423 | 1639.97 | 27 | K.KVPQVSTPTLVEVSR.N | |||||
| 410–423 | 1511.87 | 7 | K.VPQVSTPTLVEVSR.N | |||||
| 42 | Apo-serum transferrin (non-glycosylated), Chain A | 110590597 | 8 | 310–321 | 1478.72 | 44 | K.MYLGYEYVTAIR.N | |
| 310–321 | 1494.71 | 30 | K.MYLGYEYVTAIR.N | M | ||||
| 341–349 | 1195.54 | 19 | K.WCALSHHER.L | C | ||||
| 509–519 | 1283.56 | 72 | K.EGYYGYTGAFR.C | |||||
| 555–565 | 1354.62 | 33 | K.DYELLCLDGTR.K | C | ||||
| 566–578 | 1586.76 | 41 | R.KPVEEYANCHLAR.A | C | ||||
| 625–637 | 1565.78 | 18 | K.DLLFRDDTVCLAK.L | C | ||||
| 662–674 | 1531.67 | 14 | K.CSTSSLLEACTFR.R | C | ||||
| 43 | Vitamin D-binding protein/ group specific component | 455970 | 5 | 342–352 | 1388.69 | 50 | K.VMDKYTFELSR.R | |
| 342–352 | 1404.68 | 36 | K.VMDKYTFELSR.R | M | ||||
| 346–352 | 915.47 | 22 | K.YTFELSR.R | |||||
| 353–363 | 1326.76 | 38 | R.RTHLPEVFLSK.V | |||||
| 354–363 | 1170.64 | 78 | R.THLPEVFLSK.V | |||||
| 694 | Albumin, isoform CRA_h | 119626071 | 3 | 427–434 | 960.57 | 8 | K.FQNALLVR.Y | |
| 438–452 | 1639.94 | 27 | K.KVPQVSTPTLVEVSR.N | |||||
| 502–517 | 1910.93 | 17 | R.RPCFSALEVDETYVPK.E | C | ||||
| 769 | Albumin, isoform CRA_k | 119626074 | 4 | 235–242 | 960.6 | 48 | K.FQNALLVR.Y | |
| 246–260 | 1639.99 | 72 | K.KVPQVSTPTLVEVSR.N | |||||
| 247–260 | 1511.89 | 48 | K.VPQVSTPTLVEVSR.N | |||||
| 317–332 | 1910.98 | 19 | R.RPCFSALEVDETYVPK.E | C | ||||
| 770 | No Identification | – | – | |||||
| 872 | HP Protein | 47124562 | 3 | 153–161 | 980.5 | 65 | R.VGYVSGWGR.N | |
| 173–186 | 1707.82 | 95 | K.YVMLPVADQDQCIR.H | C | ||||
| 173–186 | 1723.81 | 50 | K.YVMLPVADQDQCIR.H | C, M | ||||
| 892 | Albumin, isoform CRA_h | 119626071 | 5 | 162–168 | 927.49 | 9 | K.YLYEIAR.R | |
| 427–434 | 960.56 | 35 | K.FQNALLVR.Y | |||||
| 438–452 | 1639.95 | 87 | K.KVPQVSTPTLVEVSR.N | |||||
| 439–452 | 1511.85 | 40 | K.VPQVSTPTLVEVSR.N | |||||
| 502–517 | 1910.94 | 14 | R.RPCFSALEVDETYVPK.E | C | ||||
| 900 | Haptoglobin | 3337390 | 3 | 162–168 | 927.5 | 28 | K.YLYEIAR.R | |
| 427–434 | 960.56 | 41 | K.FQNALLVR.Y | |||||
| 438–452 | 1639.93 | 33 | K.KVPQVSTPTLVEVSR.N | |||||
| 904 | Serum Albumin with S-Naproxen, Chain A | 168988718 | 7 | 6–15 | 1226.61 | 65 | R.FKDLGEENFK.A | |
| 60–68 | 1017.54 | 47 | K.SLHTLFGDK.L | |||||
| 89–101 | 1714.8 | 20 | K.QEPERNECFLQHK.D | |||||
| 132–139 | 1055.59 | 14 | K.KYLYEIAR.R | |||||
| 133–139 | 927.51 | 57 | K.YLYEIAR.R | |||||
| 333–343 | 1311.75 | 21 | R.HPDYSVVLLLR.L | |||||
| 398–405 | 960.57 | 33 | K.FQNALLVR.Y | |||||
| 5 | Albumin, isoform CRA_h | 119626071 | 4 | 162–168 | 927.5 | 28 | K.YLYEIAR.R | |
| 427–434 | 960.56 | 41 | K.FQNALLVR.Y | |||||
| 438–452 | 1639.93 | 33 | K.KVPQVSTPTLVEVSR.N | |||||
| 502–517 | 1910.93 | 18 | R.RPCFSALEVDETYVPK.E | C | ||||
| 24 | Haptoglobin | 3337390 | 3 | 217–225 | 980.52 | 61 | R.VGYVSGWGR.N | |
| 237–250 | 1707.85 | 85 | K.YVMLPVADQDQCIR.H | C | ||||
| 237–250 | 1723.83 | 55 | K.YVMLPVADQDQCIR.H | C, M | ||||
| 25 | Leucine -rich alpha-2-glycoprotein 1 | 16418467 | 3 | 165–175 | 1152.64 | 50 | K.ALGHLDLSGNR.L | |
| 210–216 | 812.48 | 18 | R.GPLQLER.L | |||||
| 251–260 | 989.58 | 45 | R.VAAGAFQGLR.Q | |||||
| 29 | Zinc-Alpha-2 Glycoprotein, Chain B | 4699583 | 4 | 44–51 | 974.49 | 5 | K.SQPMGLWR.Q | |
| 142–153 | 1532.79 | 82 | K.QKWEAEPVYVQR.A | |||||
| 156–167 | 1451.68 | 56 | K.AYLEEECPATLR.K | C | ||||
| 211–217 | 926.49 | 24 | K.IDVHWTR.A | |||||
| 30 | Complement Component C3c, Chain C | 78101271 | 4 | 62–71 | 1250.63 | 41 | K.NTMILEICTR.Y | C |
| 62–71 | 1266.61 | 11 | K.NTMILEICTR.Y | C, M | ||||
| 143–158 | 1841.98 | 120 | K.VHQYFNVELIQPGAVK.V | |||||
| 159 –171 | 1667.74 | 93 | K.VYAYYNLEESCTR.F | C | ||||
| 33 | Lipid-free Apolipoprotein A-I | 90108664 | 3 | 1–10 | 1226.57 | 34 | DEPPQSPWDR.V | |
| 161–171 | 1301.66 | 22 | R.THLAPYSDELR.Q | |||||
| 207–215 | 1012.59 | 3 | K.AKPALEDLR.Q | |||||
| 36 | Hemopexin | 11321561 | 4 | 92 –102 | 1220.61 | 64 | K.NFPSPVDAAFR.Q | |
| 198–208 | 1495.68 | 20 | R.YYCFQGNQFLR.F | |||||
| 209–219 | 1268.68 | 22 | R.FDPVRGEVPPR.Y | |||||
| 226–234 | 1142.49 | 21 | R.DYFMPCPGR.G | |||||
| 566 | Albumin, isoform CRA_k | 119626074 | 5 | 169–180 | 1467.84 | 54 | R.RHPDYSVVLLLR.L | |
| 170–180 | 1311.75 | 24 | R.HPDYSVVLLLR.L | |||||
| 235–242 | 960.57 | 43 | K.FQNALLVR.Y | |||||
| 246–260 | 1639.94 | 103 | K.KVPQVSTPTLVEVSR.N | |||||
| 247–260 | 1511.85 | 67 | K.VPQVSTPTLVEVSR.N | |||||
| 746 | Albumin, isoform CRA_p | 119626064 | 3 | 100–115 | 1910.93 | 93 | R.RPCFSALEVDETYVPK.E | C |
| 161–172 | 1342.62 | 45 | K.AVMDDFAAFVEK.C | |||||
| 161–172 | 1358.62 | 64 | K.AVMDDFAAFVEK.C | M | ||||
| 878 | No Haptoglobin, isoform CRA_d | 119579601 | 5 | 126–133 | 920.46 | 51 | K.GSFPWQAK.M | |
| 233–241 | 980.5 | 77 | R.VGYVSGWGR.N | |||||
| 253–266 | 1707.82 | 94 | K.YVMLPVADQDQCIR.H | C | ||||
| 253–266 | 1723.81 | 63 | K.YVMLPVADQDQCIR.H | C, M | ||||
| 347–356 | 1203.62 | 17 | K.VTSIQDWVQK.T | |||||
| 907 | Leucine -rich alpha-2glycoprotein 1 | 16418467 | 2 | 165–175 | 1152.63 | 30 | K.ALGHLDLSGNR.L | |
| 251–260 | 989.57 | 30 | R.VAAGAFQGLR.Q | |||||
| 976 | Albumin, isoform CRA_g | 119626070 | 3 | 35–44 | 1226.59 | 47 | R.FKDLGEENFK.A | |
| 118–130 | 1714.79 | 14 | K.QEPERNECFLQHK.D | C | ||||
| 162–168 | 927.5 | 29 | K.YLYEIAR.R | |||||
| 18 | Immunoglobulin heavy chain variable region | 39937967 | 1 | 126–137 | 1287.67 | 55 | K.GPSVFPLAPCSR.S | C |
| 236 | No Identification | – | – | |||||
| 365 | Leucine -rich alpha-2-glycoprotein 1 | 16418467 | 3 | 165–175 | 1152.62 | 57 | K.ALGHLDLSGNR.L | |
| 192–209 | 2037.08 | 125 | R.TLDLGENQLETLPPDLLR.G | |||||
| 251–260 | 989.56 | 70 | R.VAAGAFQGLR.Q | |||||
| 467 | Albumin, isoform CRA_g | 119626070 | 6 | 35–44 | 1226.62 | 81 | R.FKDLGEENFK.A | |
| 66–75 | 1149.62 | 59 | K.LVNEVTEFAK.T | |||||
| 89–97 | 1017.54 | 57 | K.SLHTLFGDK.L | |||||
| 118–130 | 1714.81 | 26 | K.QEPERNECFLQHK.D | C | ||||
| 161–168 | 1055.6 | 23 | K.KYLYEIAR.R | |||||
| 162–168 | 927.51 | 42 | K.YLYEIAR.R | |||||
| 542 | Immunoglobulin kappa 1 light chain | 170684606 | 3 | 46–61 | 1747.95 | 23 | K.LLIYDASNLETGVPSR.F | |
| 109–126 | 1946.04 | 57 | R.TVAAPSVFIFPPSDEQLK.S | |||||
| 191–207 | 1875.94 | 57 | K.VYACEVTHQGLSSPVTK.S | C | ||||
| 663 | No Identification | – | – | |||||
| 699 | Immunoglobulin light chain | 149673887 | 4 | 1–18 | 1884.05 | 63 | EIVLTQSPGTLSLSPGER.A | |
| 47–55 | 979.59 | 11 | R.LLIYGASSR.A | |||||
| 63–78 | 1632.82 | 39 | R.FSGSGSGTDFTLTISR.L | |||||
| 128–143 | 1797.92 | 73 | K.SGTASVVCLLNNFYPR.E | C | ||||
| 809 | Albumin, isoform CRA_a | 119626064 | 1 | 162–168 | 927.51 | 24 | K.YLYEIAR.R | |
| 836 | Chain A, Human Serum Transferrin, N-lobe | 29726565 | 9 | 42–50 | 1125.59 | 27 | K.KASYLDCIR.A | C |
| 43 –50 | 997.49 | 30 | K.ASYLDCIR.A | C | ||||
| 89–102 | 1629.82 | 93 | K.EDPQTFYYAVAVVK.K | |||||
| 103–113 | 1323.66 | 3 | K.KDSGFQMNQLR.G | |||||
| 104–113 | 1195.57 | 53 | K.DSGFQMNQLR.G | |||||
| 207–217 | 1273.65 | 98 | K.HSTIFENLANK.A | |||||
| 218–232 | 1881.89 | 113 | K.ADRDQYELLCLDNTR.K | C | ||||
| 221–232 | 1539.73 | 70 | R.DQYELLCLDNTR.K | C | ||||
| 240–254 | 1689.87 | 60 | K.DCHLAQVPSHTVVAR.S | C | ||||
| 865 | No Identification | – | – | |||||
| 875 | No identification | – | – | |||||
| 947 | Chain A, Human Vitamin D Binding Protein | 18655424 | 4 | 35–49 | 1694.9 | 70 | R.KFPSGTFEQVSQLVK.E | |
| 50–71 | 2518.03 | 106 | K.EVVSLTEACCAEGADPDCYDTR.T | 3C | ||||
| 79–98 | 2264.94 | 47 | K.SCESNSPFPVHPGTAECCTK.E | 3C | ||||
| 112–133 | 2707.22 | 111 | K.HQPQEFPTYVEPTNDEICEAFR.K | C |
Peptide position in the matched protein, observed mass (m/z) and amino acid sequence are listed for each protein match found.