Fig. 4.

SRI 29365 has differential efficacy in HEp-2, A549, and Vero E6 cells. Confluent monolayers of HEp-2, A549, or Vero E6 cells with either the RSVA Long strain wild-type or SRI-29356-resistant mutant in the presence of 5 μM SRI 29365, and the reduction in virus titer (TCID₅₀ reduction) was determined relative to infected (-compound) control cells (n = 4). The compound reduced wt hRSV virus TCID50 values by 300-fold, but only 10-fold in A549 cells, and less than 10-fold in Vero E6 cells. SRI 29365 reduced mutant hRSV virus TCID₅₀ values by less than 10-fold in all cell types.