| Antibodies |
| Anti-β-actin, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-47778 |
| Anti-β-tubulin, mouse monoclonal |
Sigma Aldrich |
Cat# T0198 |
| Anti-GAPDH, mouse monoclonal |
Sigma Aldrich |
Cat# G8795 |
| Anti-HSP70, mouse monoclonal |
Abcam |
Cat# Ab5439 |
| Anti-KEAP1 P586, rabbit polyclonal |
Cell Signalling |
Cat# 4678 |
| Anti-eNOS, rabbit monoclonal |
Cell Signalling |
Cat# 32027 |
| Anti-Parkin, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-136989 |
| Anti-SOD-2, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-137254 |
| Anti-TST, rabbit polyclonal |
GeneTex |
Cat# GTX114858 |
| Anti-SQRDL, rabbit polyclonal |
Sigma Aldrich |
Cat# HPA017079 |
| Anti-PTEN, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-7974 |
| Anti-PTP1B, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-133259 |
| Anti-SH-PTP2, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-7384 |
| Anti-EGFR, rabbit polyclonal |
Santa Cruz Biotechnology |
Cat# sc-03-G |
| Anti-CBS, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-133154 |
| Anti-CBS, rabbit polyclonal |
Santa Cruz Biotechnology |
Cat# sc-67154 |
| Anti-MPST, rabbit polyclonal |
Sigma Aldrich |
Cat# HPA001240 |
| Anti-CTH, rabbit |
From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) |
(Paul et al., 2014) |
| Anti-CTH, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-365382 |
| Anti- p-ERK, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-7383 |
| Anti ERK 1, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-271269 |
| Anti-DJ-1, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-55572 |
| Anti-DJ-1 AC, mouse monoclonal |
Santa Cruz Biotechnology |
Cat# sc-55572 AC |
| Anti DJ-1 (Oxidized At C106), mouse monoclonal |
BioRad |
Cat# HCA024 |
| Anti-Biotin-Peroxidase, mouse monoclonal |
Sigma Aldrich |
Cat# A0185 |
| Anti-Dimedone, rabbit polyclonal |
From Dr. Carroll’s lab (The Scripps Research Institute) |
(Seo and Carroll, 2009) |
| Mouse IgGκ BP-HRP |
Santa Cruz Biotechnology |
Cat# sc-516102 |
| Mouse anti-rabbit IgG-HRP |
Santa Cruz Biotechnology |
Cat# sc-2357 |
| Swine anti-rabbit IgG-HRP, polyclonal |
Dako, Denmark |
Cat# P0399 |
| Donkey anti-mouse IgG-HRP, polyclonal |
Abcam, Cambridge, UK |
Cat# ab6820 |
| Streptavidin Protein, DyLight 488 |
Thermo Fisher Scientific |
Cat# 21832 |
| Cy®5-Streptavidin |
Sigma Aldrich |
Cat# GEPA45001 |
| EGF Pathway Phospho Antibody Array |
Full Moon Biosystems |
Cat# PEG214 |
| Bacterial and Virus Strains |
| OP50-1 |
Caenorhabditis Genetics Center |
N/A |
| HB101 |
Caenorhabditis Genetics Center |
N/A |
|
E.coli MG1655 (pTrc99a) |
From the laboratory of Gonzales-Zorn, Spain |
N/A |
|
E.coli MG1655 (pSB74) |
From the laboratory of Gonzales-Zorn, Spain |
N/A |
|
E. coli BL21 DE3 Rosetta |
Novagen |
Cat# 70954-3 |
| Biological Samples |
| Kidneys from C57BL/6 WT and CSE−/− mice |
From Professor Maik Gollasch (Charité Medical Faculty) |
(Markó et al., 2016) |
|
D. melanogaster: y1w1118
|
From Professor Ody Sibon (University of Groningen) |
(Snijder et al., 2015) |
|
D. melanogaster: Eip55E |
From Professor Ody Sibon (University of Groningen) |
(Snijder et al., 2015) |
| Chemicals, Peptides and Recombinant Proteins |
| Chelex-100 |
Sigma Aldrich |
Cat# C7901 |
| Sodium Sulfide |
Sigma Aldrich |
Cat# 407410 |
| GYY4137 |
(Alexander et al., 2015) |
N/A |
| AP39 |
(Le Trionnaire et al., 2014) |
N/A |
| D-Cystine |
Sigma Aldrich |
Cat# 30095 |
| DL-propargylglycine |
Sigma Aldrich |
Cat# P7888 |
| O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOAA) |
Sigma Aldrich |
Cat# C13408
|
| Hydrogen peroxide solution |
Sigma Aldrich |
Cat# 216763 |
| Monensin sodium salt |
Sigma Aldrich |
Cat# M5273-1G |
| Erastin |
Sigma Aldrich |
Cat# E7781 |
|
S-Sulfocysteine |
Sigma Aldrich |
Cat# C2196 |
| Cystine |
Sigma Aldrich |
Cat# 30200 |
| Imidazole |
Carl Roth Gmbh |
Cat# 3899.4 |
| Isopropyl β-D-1-thiogalactopyranoside |
Inalco Pharmaceuticals |
Cat# 1758-1400 |
| Dithiothreitol |
GE Healthcare |
Cat# 17-1318-02 |
| 4-Chloro-7-nitrobenzofurazan |
Sigma Aldrich |
Cat# 163260 |
| Dimedone |
Sigma Aldrich |
Cat# D153303 |
| DCP-Bio1 |
Kerafast |
Cat# EE0028 |
| DAz-2 |
Cayman Chemicals |
Cat# 13382 |
| BioDiaAlk |
(Akter et al., 2018) |
N/A |
| DiaAlk |
(Akter et al., 2018) |
N/A |
| Cyanine5 alkyne |
Lumiprobe |
Cat# FP-OO5590 |
| Copper(II)-TBTA |
Lumiprobe |
Cat# 21050 |
| L-Ascorbic acid |
Sigma Aldrich |
Cat# 795437 |
| 5-Fluoro-2′-deoxyuridine |
Sigma Aldrich |
#F0503 |
| Nmc-penicillamine |
(Artaud and Galardon, 2014) |
N/A |
| EGF human recombinant |
PromoKine |
Cat# 60170 |
| VEGF-165, human, recombinant |
PromoCell |
Cat# C-64422 |
| Insulin human |
Sigma Aldrich |
Cat# I0908 |
| Paraformaldehyde |
Sigma Aldrich |
Cat# P6148 |
| Sodium thiosulfate |
PROLABO |
Cat# 27 910.291 |
| Paraquat hydrochloride hydrate |
Sigma Aldrich |
Cat# 36541 |
| Sodium (meta)arsenite |
Sigma Aldrich |
Cat# S7400 |
| 2-Deoxy-D-glucose |
Sigma Aldrich |
Cat# D8375 |
| Thiazolyl Blue Tetrazolium Bromide |
Sigma Aldrich |
Cat# M5655 |
| Protease Inhibitor |
Sigma Aldrich |
Cat# P8340 |
| His-Pur Ni-NTA Superflow Agarose |
Thermo Fisher Scientific |
Cat# 25216 |
| Streptavidin Magnetic Beads |
Sigma Aldrich |
Cat# 11 641 778 001 |
| NeutrAvidin Agarose Resin |
Thermo Fisher Scientific |
Cat# 29201 |
| High Capacity NeutrAvidin Agarose Resin |
Thermo Fisher Scientific |
Cat# 29202 |
| Trypsin from porcine pancreas |
Sigma Aldrich |
Cat# T6567 |
| Chymotrypsin Sequencing Grade |
Sigma Aldrich |
Cat# 000000011418467001 |
| Human Serum Albumin (HSA) |
Sigma Aldrich |
Cat# A1887 |
| GAPDH |
Sigma Aldrich |
Cat# G2267 |
| Rhodanese from bovine liver (TST) |
Sigma Aldrich |
Cat# R1756 |
| MnSOD |
Creative BioMart |
Cat# SOD2-1039H |
| Cytochrome c |
Sigma Aldrich |
Cat# 30398 |
| Xanthine |
Sigma Aldrich |
Cat# X0626 |
| PTP1B human recombinant |
Abcam, Cambridge, UK |
Cat# ab51277 |
| Xanthine Oxidase |
Sigma Aldrich |
Cat# X1875 |
| Thioredoxin Reductase (TrxR) from rat liver |
Sigma Aldrich |
Cat# T9698 |
| TRP14 (From human fibroblast cDNA) |
For this paper |
N/A |
|
hsTrx C32S |
For this paper |
N/A |
|
hsTrx C35S |
For this paper |
N/A |
|
hsTrx1 |
For this paper |
N/A |
| NADPH |
Sigma Aldrich |
Cat# N5130 |
| DAPI |
Euromedex |
Cat# 1050-A |
| MeRho-Az |
From Michael D. Pluth’s lab (Dept. of Chemistry and Biochemistry, Eugene, OR) |
N/A |
| Propidium Iodide |
Sigma Aldrich |
Cat# P4864 |
| Histowax® |
Histolab Product AB, Sweden |
Cat# 00405 |
| Normal swine serum |
Dako, Denmark |
Cat# X0901 |
| Normal donkey serum |
Abcam, Cambridge, UK |
Cat# X0903 |
| 3,3′-Diaminobenzidine tetrahydrochloride (DAB) |
Dako North America, Inc. Carpintería, CA, USA |
Cat# K3468 |
| DPX medium |
Sigma-Aldrich |
Cat# 06522 |
| Experimental Models: Cell Lines |
| Human: HeLa |
ECACC |
Cat# 93021013 |
| Human: HUVEC |
PromoCell |
Cat# C-12203 |
| Human: SH-SY5Y |
ECACC |
Cat# 94030304 |
| Human: Fibroblasts (male) |
Coriell Institute |
AG08790 and AG14245 |
| Mouse: MEF WT (WT) (male) |
From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) |
(Sbodio et al., 2016) |
| Mouse: MEF CSE−/− (male) |
From Prof. Snyder’s lab (Johns Hopkins University School of Medicine) |
(Sbodio et al., 2016) |
| Mouse: Striatal progenitor cells STHdhQ7/Q7 (male) |
M. MacDonald (Massachusetts General Hospital, Boston, MA) |
N/A |
| Mouse: Striatal progenitor cells STHdhQ111/Q111 (male) |
M. MacDonald (Massachusetts General Hospital, Boston, MA) |
N/A |
| Experimental Models: C.elegans mutants |
| Wild-type Bristol N2 |
Caenorhabditis Genetic Center |
N/A |
| cth-1(ok3319)V |
Caenorhabditis Genetic Center |
VC2569 |
| mpst-3(tm4387)V |
MITANI Lab, National Bio-Resource Project of the MEXT, Japan |
FX04387 |
| eat-2(tm5786)II |
MITANI Lab, National BioResource Project of the MEXT, Japan |
FX19451 |
| eat-2(tm5786)II;cth-1(ok3319)V |
This paper |
N/A |
| eat-2(tm5786)II;mpst-3(tm4387)V |
This paper |
N/A |
| Experimental Models: Strains/Organisms |
|
S. cerevisiae: BY4742 (WT) |
Euroscarf |
N/A |
|
S. cerevisiae: Δcys4
|
Euroscarf |
ACCNO Y16696
|
|
S. cerevisiae: Δcys3
|
Euroscarf |
ACCNO Y16865
|
|
S. cerevisiae: Δtum1
|
Euroscarf |
ACCNO Y12507
|
| Male Wistar Rat |
Institute for Biological Research “Siniša Stanković”, Belgrade, Serbia |
N/A |
| Male C57BL/6J mice |
NIA Aging Colony Resource at Charles River Laboratories (CRL) |
N/A |
| Male C57BL/6 mice |
Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD |
N/A |
| Oligonucleotides |
| 5’-GAAGGAGATATACCATGGTGAAGC-3’ (fwd) |
Sigma Aldrich |
hsTrx in pET28a |
| 5’-CGGATCTCAGTGGTGGTG-3’ (rev) |
Sigma Aldrich |
hsTrx in pET28a |
| 5’-CACGTGGTCTGGGCCTTG-3’ (fwd) |
Sigma Aldrich |
hsTrx C32S mutagenesis |
| 5’-CAAGGCCCAGACCACGTG-3’ (rev) |
Sigma Aldrich |
hsTrx C32S mutagenesis |
| 5’-GTGTGGGCCTTCCAAAATGATCAAG-3’ (fwd) |
Sigma Aldrich |
hsTrx C35S mutagenesis |
| 5’-CTTGATCATTTTGGAAGGCCCACAC-3’ (rev) |
Sigma Aldrich |
hsTrx C35S mutagenesis |
| 5’-ACCATCACGGATCCATGGCCCGCTATGAGGAGG-3’ (fwd) |
Sigma Aldrich |
hsTRP14 in pQE-80L |
| 5’-CCGGGGTACCGTTAATCTTCAGAGAACAACATTTCCACCAG-3’ (rev) |
Sigma Aldrich |
hsTRP14 in pQE-80L |
| Recombinant DNA |
| Plasmid: pET-28a(+) |
Novagen |
Cat# 69864-3 |
| Plasmid: pQE-80L |
Qiagen |
N/A |
| Software and Algorithms |
| ImageJ |
NIH |
https://imagej.nih.gov/ij/download.html |
| GraphPad Prism 5.0 |
GraphPad Software |
https://www.graphpad.com |
| PEAKS Studio |
Bioinformatics Solutions Inc. |
http://www.bioinfor.com/ |
| OriginPro 8 |
OriginLab |
https://www.originlab.com/ |
| RTCA Software Version 2.0 |
ACEA Biosciences, Inc. |
https://www.aceabio.com/products/rtca-dp/ |
| CFLow Plus Version 1.0.202.1 |
BD Biosciences |
http://www.bdbiosciences.com |
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