Table 1.
Primers used to amplify Cryptosporidium spp. 18S rRNA, gp60, and hsp70 gene fragments in the diarrheal feces of young calves.
| Target species | Target gene | Primer name | Primer sequence (5′ to 3′) | Tm (°C) | Reference |
|---|---|---|---|---|---|
| Cryptosporidium spp. | 18S rRNA | 18SiF | AGT GAC AAG AAA TAA CAA TAC AGG | 55.8 | Cheun et al. (2007) |
| 18SiR | CCT GCT TTA AGC ACT CTA ATT TTC | 57.5 | |||
| hsp70 | CPHSPF | AGT GAT ATG ACT CAC TGG CCA TT | 58.8 | Satoh et al. (2005) | |
| CPHSPR | ACA ACA TCA TGT ACA GAT CTC TT | 55.3 | |||
| C. parvum | 18S rRNA | CpaFca | GAA ATA ACA ATA CAG GAC TTT TT | 51.7 | In this study |
| gp60 | AL3531 | ATA GTC TCC GCT GTA TTC | 51.4 | Peng et al. (2001) | |
| AL3534 | GCA GAG GAA CCA GCA TC | 55.1 | |||
| AL3532 | TCC GCT GTA TTC TCA GCC | 55.6 | Peng et al. (2001) | ||
| AL3533 | GAG ATA TAT CTT GGT GCG | 44.4 | |||
| C. andersoni | 18S rRNA | Can18Fa | ACG GAT CGC ATC TCT GAT GC | 59.3 | In this study |
| C. bovis/ryanae | 18S rRNA | Cbrx18Fa | CAA TAC AGA RCC TTA CGG TT | 56.4 | In this study |
a
18SiR was used as the reverse primer.