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. 2016 Sep 28;229:81–89. doi: 10.1016/j.vetpar.2016.09.019

Table 3.

Detection of Cryptosporidium parvum and C. bovis/ryanae using PCR analysis and ELISA.

Group
No. tested C. parvum
C. bovis/ryanae
PCR
ELISA
PCR
No. positive (%) 95% CIa P-value No. positive (%) 95% CIa P-value No. positive (%) 95% CIa P-value
Sex Male 433 21 (4.8) 2.8–6.9 0.312 42 (9.7) 6.9–12.5 0.370 15 (3.5) 1.7–5.2 0.911
Female 248 8 (3.2) 1.0–5.4 19 (7.7) 4.4–11.0 9 (3.6) 1.3–6.0
Unknown 270 29 (10.7) 7.1–14.4 31 (11.5) 7.7–15.3 15 (5.6) 2.8–8.3



Type of cattle Beef 909 56 (6.2) 4.6–7.7 0.529 90 (9.9) 8.0–11.8 0.954 38 (4.2) 2.9–5.5 0.895
Dairy 21 2 (9.5) 0–22.1 2 (9.5) 0–22.1 1 (4.8) 0–13.9
Unknown 21



Region Northern 222 3 (1.4) 0–2.9 <0.001 10 (4.5) 1.8–7.2 <0.001 5 (2.3) 0.3–4.2 0.059
Central 146 26 (17.8) 11.6–24.0 29 (19.9) 13.4–26.3 3 (2.1) 0–4.4
Southern 583 29 (5.0) 3.2–6.7 53 (9.1) 6.8–11.4 31 (5.3) 3.5–7.1



Season Spring
(Mar–May)
252 15 (6.0) 3.0–8.9 0.854 18 (6.7) 3.7–9.8 0.158 2 (0.8) 0–1.9 <0.001
Summer
(Jun–Aug)
240 13 (5.4) 2.6–8.3 22 (9.2) 5.5–12.8 18 (7.5) 3.5–9.8
Fall
(Sep–Nov)
250 18 (7.2) 4.0–10.4 25 (10.0) 6.3–13.7 13 (5.2) 2.5–8.0
Winter
(Dec–Feb)
209 12 (5.7) 2.6–8.9 28 (13.4) 8.8–18.0 6 (2.9) 1.2–6.4



Type of diarrhea Hemorrhagic 105 0 0.025 1 (1.0) 0–2.8 0.004 10 (9.5) 2.5–12.7 0.008
Watery 419 27 (6.4) 4.1–8.8 49 (11.7) 8.6–14.8 17 (4.1) 2.2–6.0
Pasty 427 31 (7.3) 4.8–9.7 42 (9.8) 7.0–12.7 12 (2.8) 1.6–5.0
Total 951 58 (6.1) 4.6–7.6 92 (9.7) 7.8–11.6 39 (4.1) 2.5–5.4
a

CI: Confidence interval.