Table 1.
Primers pairs used to amplify the S1 gene hypervariable region and S gene fragment.
| Name | Primer sequence (5′–3′) | Location (BCoV complete genome) | Amplicon size (basepairs) | References |
|---|---|---|---|---|
| S1HS | CTATACCCAATGGTAGGA | 24827–24844 | 885 bp | Brandao et al. (2006) |
| S1HA | CTGAAACACGACCGCTAT | 25694–25711 | ||
| S1NS | GTTTCTGTTAGCAGGTTTAA | 24952–24971 | 488 bp | Brandao et al. (2006) |
| S1NA | ATATTACACCTATC CCCTTG | 25420–25439 | ||
| S1For | TTGTAATTTTAATATGAGCAGCC | 24808–24830 | 908 pb | In this study |
| S1Rev | TTCTGCCAACTATTATAATAAG | 25695–25716 | ||
| S2For | TTATAATAGTTGGCAGAACC | 25699–25718 | 672 pb | In this study |
| S2Rev | ACCATTCATTAAACTATTAGC | 26351–26371 |
Primers pairs used for amplification of S1 hypervariable region: S1HS, S1HA, S1NS, S1NA, primers pairs used for amplification of S gene fragment: S1For, S1Rev, S2for, S2 Rev.