Table 3.
Amino acid substitutions in the studied S gene portion.
| Portion of 508 aa (1453 pb) used for characterization (356–873 aa) | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 359 | 455 | 458 | 465 | 470 | 484 | 492 | 494 | 499 | 501 | 525 | 531 | 533 | 543 | 545 | 578 | 716 | 740 | 751 | 769 | 778 | 785 | 853 | |
| Mebus/1983 | N | T | F | V | H | S | D | S | N | P | H | N | L | S | G | T | T | S | T | A | T | N | A |
| Arg95a | Y | I | S | A | D | T | – | – | S | S | Y | D | M | A | W | S | P | I | P | S | N | – | – |
| Argentinean strains b | Y | I | S | A | D | T | N | A | S | S | Y | D | M | A | W | – | – | – | – | S | N | K | S |
Numbers are in concordance with Spike gene of Mebus reference strain (Accesion number U00735).
In bold, hypervariable region (461–570 aa) of 109 aa used for diagnostic PCR are shown. Amino acid identity marked as -:
a
Argentinean tissue culture cell adapted virus.
b
Consensus of Argentinean field strains.