Table 1.
Sequences of oligonucleotide primers used in this study.
| Targate | Primer name | Sequence (5′-3′) | Product (bp) | Reference | |
|---|---|---|---|---|---|
| Partial 3D gene | UNIV-Kobu-F | TGGAYTACAAGTGTTTTGATGC | 216 | Reuter et al. 2009 | |
| UNIV-Kobu-R | ATGTTGTTRATGATGGTGTTGA | ||||
| FeKoV-3D-F | CTCCGCCCCACCGCTAAGG | 530 | This study | ||
| FeKoV-3D-R | GGGGGTTCCGTTGCGTAGATGA | ||||
| Complete VP1 gene | FeKoV-VP1-F | GCCCCCGCCTCTGCCATTGTG | 843 | This study | |
| FeKoV-VP1-R | CTTGATGACGGCGACGGACTTTTC | ||||
| Complete polyprotein genea | 5′UTR-VP0 | FeKoV-535-F | GTTCGTCCGGCTGTCCTTTGGTAA | 1164 | This study |
| FeKoV-1698-R | AGTAGCRGTGGGGTAGGCRAGRAA | ||||
| VP0-VP3 | FeKoV-1601-F | GCCTGGCYGCYCTCAATCCTTCA | 1127 | ||
| FeKoV-2727-R | GGGGGCAGCGGGGGTGTAGC | ||||
| VP3-2B | FeKoV-2567-F | TCCCCGTCTCCCCCAGTGCYATTG | 1654 | ||
| FeKoV-4220-R | GTGTCGGCGTCGGCGGTCAG | ||||
| 2B-2C | FeKoV-3753-F | AAGTCCGTCGCCGTCATCAAGA | 1330 | ||
| FeKoV-5087-R | GTCCCGGGCGGTCCATAGAAAT | ||||
| 2C-3D | FeKoV-4948-F | AGGATGCAAAATCTGGCCCACACTCTG | 1788 | ||
| FeKoV-6735-R | GGGGGCAGGTTCCTTCTTGATGG | ||||
| 3D-3′UTR | FeKoV-6503-F | TCCCCGCTTGTGACAGATGACC | 1672 | ||
| FeKoV-8155-R | TACAACCATGGCTTAGGGGCTCAC | ||||
a
The primers' positions are referred to the full-length genome of FeKoV reference strain 12D240 (Genbank accession number: KJ958930).