Fig. 2.

IL-2 and/or IL-10 addition helped TCR-stimulated CD8± T cells to maintain their cytotoxicity. The MCF-7 breast cancer cell line was labeled with Cr-51 and was used as target cells. 0% specific lysis corresponds to the spontaneous Cr-51 release level and 100% specific lysis corresponds to the Cr-51 release level when 1% Triton X-100 was used to lyse all target cells. (A) Short-term (24 h) TCR-stimulated CD8⁺ T cells in None, +IL-2, +IL-10, or +IL-2 + IL-10 condition were used as effector cells. (B) Long-term (72 h) TCR-stimulated CD8⁺ T cells in None, +IL-2, +IL-10, or +IL-2 + IL-10 condition were used as effector cells. The statistical difference between each experimental group and the corresponding None control group was examined using two-way ANOVA followed by Dunnett’s test. Bars represent SEM. ns: not significant. *P < 0.05. **P < 0.01. ***P < 0.001.