Fig. 3.

IL-2 and/or IL-10 addition increased the proliferation of TCR-stimulated CD8± T cells. CD8⁺ T cells in CD4-depleted PBMCs were labeled with CFSE and TCR-stimulated with anti-CD3/anti-CD28 monoclonal antibodies (3 μg/mL each) for 72 h and were harvested for flow cytometry examination. (A) The percentage of dividing cells (bold numbers) was gated as CFSE-low cells. Panels shown were already gated on CD3⁺CD4⁻CD8⁺ T cells. (B) The frequencies of dividing cells in None, +IL-2, +IL-10, and +IL-2 + IL-10 conditions after 72 h TCR-stimulation of CD8⁺ T cells. (C) The number of CD8⁺ T cells, counted in flow cytometer, at the end of 72 h TCR-stimulation, starting from 10⁶ CD8⁺ T cells per condition. The statistical difference between each experimental group and the corresponding None control group was examined by one-way ANOVA followed by Dunnett’s test. Bars represent SEM. ns: not significant. *P < 0.05. ***P < 0.001.