Table 1.

(a) Feline coronavirus (FCoV) primers and probe for the FCoV fluorogenic assay; (b) position of primers and probe in the sequence of the FCoV 7b gene

Primer or probe		Sequence (5′→3′)		Length of fragment (bp)
(a)
Forward primer FcoV1128f		GAT TTG ATT TGG CAA TGC TAG ATT T
Reverse primer FcoV1229r		AAC AAT CAC TAG ATC CAG ACG TTA GCT		102
Probe FCoV1200p		aTCC ATT GTT GGC TCG TCA TAG CGG Ab
(b)
1001	GGCAACCCGA	TGTCTAAAAC	TGGTCTTTCC	GAGGAATTAC	GGGTCATCGC
1051	GCTGCCTACT	CTTGTACAGA	ATGGTAAGCA	CGTGTAATAG	GAGGTACAAG
				FCoV1128→
1101	CAACCCTATT	GCATATTAGG	AAGTTTAGAT	TTGATTTGGC	AATGCTAGAT
				←FCoV1200p
1151	TTAGTAATTT	AGAGAAGTTT	AAAGATCCGC	TATGACGAGC	CAACAATGGA
		←FCoV1229r
1201	AGAGCTAACG	TCTGGATCTA	GTGATTGTTT	AAAATGTAAA ATTGTTTGAA
1251	AATTTTCCTT	TTGATAGTGA	TG

^aReporter dye (FAM) labeled nucleotide.

^bQuencher dye (TAMRA) labeled nucleotide.