Table 2.

Comparison of Ct values after amplification of dilution series of titrated virus in mono-specific and multiplex real-time PCR

	Ct value (individual PCR)	Ct value (multiplex)	Ct value (multiplex with EAV spike)
Rhinovirus
100 TCID50	26.2	26.5	26.3
10 TCID50	29.4	29.3	29.7
1 TCID50	33.1	33.1	33.4
0.1 TCID50	36.5	36.3	37.1
0.01 TCID50	40.2	40.5	40.9
0.001 TCID50	0	0	0
Metapneumovirus
100 TCID50	19.2	19.1	19.7
10 TCID50	23.1	23.3	23.5
1 TCID50	27.3	27.1	27.9
0.1 TCID50	31.2	31.2	32.0
0.01 TCID50	36.4	38.1	38.2
0.001 TCID50	0	0	0

For each virus a 10-fold dilution series is shown with Ct values. For each virus the lowest level of detection is shown.