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. 2008 Feb 2;866(1):123–132. doi: 10.1016/j.jchromb.2008.01.029

Fig. 4.

Fig. 4

(a) Separation chromatogram of rat liver tissue lysate proteins by 1D SCX. Fractions were collected every 2 min automatically from 3 to 127 min, and collection time of fraction was demonstrated in the magnified illustration. (b) The RPLC elution profile of SCX fraction 3. (c) 3D display of SCX–RPLC separation of rat liver proteins sample. In total, 62 fractions were obtained from the first dimensional separation of SCX, which were separated by RPLC further. Positions of part high-abundance proteins were as labeled [68].