Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Mar 28;18(5):741–753. doi: 10.1093/intimm/dxl011

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

PMC Copyright notice

Fig. 3. — CHO cells expressing CIRE/mDC-SIGN bind mannosylated protein in a calcium-dependent, mannan-inhibitable manner. CHO cells (10⁵) expressing FLAG-CIRE/mDC-SIGN (FLAG-CIRE-CHO), CIRE/mDC-SIGN (CIRE-CHO) or neomycin resistance gene only (Neo-CHO) were re-suspended in various dilutions of mannan in ice-cold medium containing 2% FCS and Ca²⁺ and Mg²⁺ or in medium where the Ca²⁺ and Mg²⁺ were replaced by 5 mM EDTA. (A) Mannan and EDTA inhibit the binding of mannosylated FITC-conjugated BSA to CIRE/mDC-SIGN. (B) Mannan inhibits the binding of mannosylated FITC-conjugated BSA by CIRE/mDC-SIGN in a dose-dependent manner. Data are representative of a minimum of five independent experiments. (C) Human DC-SIGN and mouse SIGNR1 also bind mannosylated FITC-conjugated BSA in a Ca²⁺-dependent manner. Cells were then incubated with mannosylated FITC-conjugated BSA and fluorescence levels quantitated by flow cytometry. Data are representative of two independent experiments.