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. 2006 Mar 28;18(5):741–753. doi: 10.1093/intimm/dxl011

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© The Japanese Society for Immunology. 2006. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

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Fig. 4. — CIRE/mDC-SIGN⁺ DC does not preferentially activate T cells into proliferation. DCs from SPF mice were isolated and then sorted based on their expression of CD11c⁺CIRE/mDC-SIGN⁺ (purity: 75%) and CD11c⁺CIRE/mDC-SIGN⁻ (purity: 98%). DCs were incubated with 20 000 CD4 or CD8 T cells for 4 and 3 days, respectively, then pulsed with 1 μCi per well of [³H]thymidine for 6 h, harvested onto glass-fibre filters and thymidine incorporation was counted by liquid scintillation. All cultures were performed in quintets and are representative of two independent experiments.