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. 2006 Mar 28;18(5):741–753. doi: 10.1093/intimm/dxl011

Fig. 6.

Fig. 6.

The chimeric molecule containing the CIRE/mDC-SIGN lectin domain fused to human DC-SIGN stalk appears to form multimers similarly to hDC-SIGN. Cell lysates from parental CHO cells (lane 1) or CHO cells expressing hDC-SIGN (lane 2), or the chimeric molecules [CIRE/mDC-SIGN lectin fused to hDC-SIGN stalk (lane 3) and hDC-SIGN lectin fused to CIRE/mDC-SIGN stalk (lane 4)] and CIRE/mDC-SIGN (lane 5) were separated on SDS-PAGE (10%) under reducing and non-reducing conditions, probed with mouse anti-human DC-SIGN mAb and anti-CIRE/mDC-SIGN and then revealed using anti-rat–HRP and anti-mouse–HRP antibody.