Table 3.
Evaluation of the specificity of mAbs against Influenza A viruses by indirect ELISA (immobilised antigen), sandwich ELISA (antigen in solution) and western blot.
| mAb no. | Indirect ELISA |
Sandwich ELISAa |
Western blot |
|||
|---|---|---|---|---|---|---|
| H5 virusesb | Other virusesb | H5 virusesb | Other virusesb | H5 virusesb | Subtype H1 virus | |
| 20/1/E6 | 10/11 | 16/19 | 7/11 | 10/19 | 11/11 | 1/1 |
| 107/1/E4 | 7/11 | 15/19 | 8/11 | 6/19 | 11/11 | 1/1 |
| 140/1/D9 | 7/11 | 13/19 | 9/11 | 4/19 | 11/11 | 1/1 |
| 10/1/D6 | 9/11 | 4/19 | 8/11 | None | 8/11 | 1/1 |
| 1/03/F2 | 10/11 | None | 7/11 | Nonec | 10/11 | 0/1 |
| 23/3/G5 | 10/11 | None | 4/11 | Nonec | 9/11 | 0/1 |
| 492/3/E6 | 9/11 | None | 8/11 | None | 9/11 | 0/1 |
| 21/03/D2 | 8/11 | None | 5/11 | Nonec | 9/11 | 0/1 |
| 157/1/F4 | 9/11 | None | 8/11 | None | 10/11 | 0/1 |
| 132/03/D8 | 7/11 | None | 5/11 | Nonec | 8/11 | 1/1 |
| 22/03/D9 | 6/11 | None | 6/11 | Nonec | 9/11 | 0/1 |
Given are the number of virus isolates detected by the mAb and the number of viruses tested, respectively. mAb = monoclonal antibody.
a
Capture Ab = rabbit anti-H5N1 polyclonal antibody, detector Ab = biotinylated mAb.
b
See Table 1.
c
Reactions with other influenza A viruses were only analysed for subtypes H1, H3, and H7.