Fig. 4.

Assessment of cellular lysosomal enzyme activity during phagocytosis. RAW264.7 cells with various concentrations of thymol (50, 100 and 150 μM) as well as with PBS for control group were incubated in 96-well plates for 24 h at 37 °C. Measurement of acid phosphatase activity was done by using p-nitrophenyl phosphate (p-NPP) solution. The phagocytic stimulation index (SI) was calculated as the absorbance ratio of the treated system and the control system. Results are depicted as the mean ± standard error of the mean (SEM). Statistical significance was calculated using the Students t-tests. ** represents P-value < 0.01.