Fig. 6.

Effect of thymol on cytokines level. The RAW264.7 cells were cultured with various concentration of thymol (50,100 and 150 μM) for 12 h after the incubation period a further treatment with LPS (100 ng/ml) and PBS as a control was done and cells were incubated for 6 h of period. Amount of various cytokines in supernatant was measured by ELISA. (A) TNF α, (B) IL-1ß and (C) Prostaglandin E2. Results are depicted as the mean ± standard error of the mean (SEM). Statistical significance was calculated using the Students t-tests. * represents P-value < 0.05, *** represents P-value < 0.001 and # represents P-value < 0.001.