Fig. 7.

Anticomplementary activity of thymol. Antibody sensitized sheep red blood cells (SRBC) were used as an indicator to determine anticomplementary activity of thymol. Diluted complement source, sensitized sheep red blood cells and different concentration of thymol were added in a round bottom microtiter plate and were incubated for 30 min 37 °C. Degree of hemolysis was determined and percentage of inhibition was calculated. Rosmarinic acid (100 μM) was used as a positive control. Results are depicted as the mean ± standard error of the mean (SEM). Statistical significance was calculated using the Students t-tests. * represents P-value < 0.05 and *** represents P-value < 0.001.