Table 2.
Aspergillus fumigatus Bioevolution and MycoGENIE A. fumigatus Ademtech (CT) RT-PCR Assay, Culture, and Serologic Characteristics for 31 BAL Samples of Patients with Probable Invasive Pulmonary Aspergillosis
| BAL |
Serum galactomannan result | Other Aspergillus-positive culture | Other pathogens in the BAL sample | |||||
|---|---|---|---|---|---|---|---|---|
| Sample no. | Real-time A. fumigatus PCR kit (Ct) | MycoGENIE (Ct) | Culture | Direct examination. | Galactomannan index | |||
| 1 | Positive (28) | Positive (26) | A. fumigatus >40 colonies | Hyphae | >5 | Positive | Tracheal aspiration | None |
| 2 | Positive (35) | Positive (33) | Negative | Negative | 0.99 | Negative | Tracheal aspiration | CMV, Coronavirus |
| 3 | Positive (35) | Positive (34) | A. fumigatus 10–20 colonies | Negative | 3.47 | Positive | Bronchial lavage | None |
| 4 | Positive (22) | Positive (21) | A. fumigatus >40 colonies | Hyphae | >5 | Positive | Tracheal aspiration | None |
| 5 | Negative | Negative | Candida albicans 1 colony | Negative | >5 | Negative | None | None |
| 6 | Negative | Negative | Negative | Negative | 1.59 | Negative | None | Enterovirus |
| 7 | Positive (35) | Positive (34) | A. fumigatus 5 colonies | Hyphae | 0.07 | Negative | None | Influenza A (H1N1) |
| 8 | Positive (34) | Positive (31) | Negative | Negative | >5 | Positive | None | None |
| 9 | Negative | Negative | Negative | Negative | 0.79 | Negative | None | Pseudomonas aeruginosa |
| 10 | Positive (35) | Negative | C. albicans >40 colonies | Negative | 1.12 | Negative | None | Staphylococcus epidermidis, Pneumocystis |
| 11 | Positive (35) | Positive (31) | C. albicans 10–20 colonies | Negative | 0.24 | Negative | Tracheal aspiration | CMV, Pneumocystis |
| 12 | Positive (35) | Positive (39) | C. albicans >40 colonies | Negative | 0.74 | Negative | None | Pneumocystis |
| 13 | Negative | Negative | Penicillium species 1 colony | Negative | 0.61 | Negative | None | Stenotrophomonas maltophilia |
| 14 | Negative | Negative | Negative | Negative | 1.40 | Positive | None | None |
| 15 | Positive (35) | Negative | A. fumigatus 1 colony | Negative | 0.76 | Negative | None | Enterococcus faecium, Pneumocystis |
| 16 | Positive (35) | Positive (34) | Negative | Negative | >5 | Positive | None | CMV |
| 17 | Positive (33) | Positive (31) | A. fumigatus 1 col., A. niger 3 col. | Negative | >5 | Positive | Subsequent BAL | Pneumocystis |
| 18 | Positive (28) | Positive (26) | A. fumigatus 6 colonies, A. niger 1 colony, Trichosporon asahii >40 colonies | Hyphae | >5 | Positive | None | Pneumocystis, Influenza |
| 19 | Positive (30) | Positive (28) | A. fumigatus 20–40 colonies | Hyphae | >5 | Negative | None | Influenza A (H1N1), Pseudomonas aeruginosa |
| 20 | Positive (35) | Negative | A. fumigatus 6 colonies | Negative | >5 | Negative | Tracheal aspiration | Staphylococcus aureus |
| 21 | Positive (25) | Positive (23) | A. fumigatus 20–40 colonies | Hyphae | >5 | Negative | Tracheal aspiration | None |
| 22 | Positive (35) | Positive (33) | Negative | Hyphae | 0.18 | Negative | None | Enterococcus faecium |
| 23 | Negative | Negative | Negative | Hyphae | 0.59 | Negative | None | Enterococcus faecium, Coronavirus, Pneumocystis |
| 24 | Positive (34) | Positive (31) | A. fumigatus 1 colony | Hyphae | 0.48 | Negative | Bronchial aspiration | None |
| 25 | Positive (35) | Positive (33) | Negative | Negative | >5 | Positive | None | Enterococcus faecium |
| 26 | Positive (35) | Positive (32) | Negative | Negative | >5 | Positive | Stool | None |
| 27 | Positive (32) | Positive (30) | A. fumigatus 1 colony | Negative | 2.75 | Negative | Prior BAL | Parainfluenza virus |
| 28 | Positive (28) | Positive (26) | A. fumigatus >40 colonies | Negative | >5 | Positive | None | Pseudomonas aeruginosa |
| 29 | Positive (35) | Positive (33) | Negative | Negative | 2.11 | Positive | Sputum | Stenotrophomonas maltophilia, Parainfluenza virus |
| 30 | Positive (35) | Positive (32) | Negative | Negative | >5 | Positive | None | None |
| 31 | Positive (31) | Positive (28) | A. fumigatus 5 colonies | Negative | 0.61 | Negative | Tracheal aspiration | None |
BAL, bronchoalveolar lavage; CMV, cytomegalovirus.