Figure 1.

A, TGFβ signaling in DCs is reduced in T-cell-mediated colitis. CD4⁺ CD45RB^hi-naïve T cells were adoptively transferred into Rag1^-/- mice to induce colitis. PBS-injected mice served as the control group. At the end of the experiment, mice were killed, and single cells from the colonic lamina propria were subjected to flow cytometry to evaluate pSmad2/3 expression in conventional (CD11c^hi PDCA1^-) and plasmacytoid DCs (CD11c^lo PDCA1+). Histograms show decreases in pSmad2/3 expression in conventional but not plasmacytoid DCs. B, Dendritic cells from Rag^-/-Cre⁺Tgfbr2^ΔDC mice are not activated at steady state. Surface expression of MHCII, CD80, CD86, CD40, and E-cadherin on the conventional and plasmacytoid DCs from MLNs of Rag1^-/- Cre^- and Rag1^-/-Tgfbr2^ΔDC mice. Three to 4 mice from each genotype were pooled to get a sufficient number of cells. The histograms are representative of 2 independent experiments.