Figure 3.

Both CD4⁺ and CD8⁺ T cells are necessary to induce colitis in Rag1^-/-Tgfbr2^ΔDC mice. Colitis did not develop in Rag1^-/-Tgfbr2^ΔDC mice adoptively transferred with CD4⁺ or CD8⁺ T cells alone (n = 5–8 mice per genotype). A–B, Weight loss curves for mice transferred with CD4⁺ or CD8⁺ T cells. C, H&E staining of colonic segments representative of each experimental group. D–E, qPCR analysis of colonic mucosal expression of selected cytokines in mice transferred with CD4⁺ or CD8⁺ T cells. Data were analyzed using the 2^-∆∆CT method with TBP used as a housekeeping gene. The unpaired 2-tailed t test was used to analyze the data. *P < 0.05.