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. 2020 Apr 27;15(4):e0232047. doi: 10.1371/journal.pone.0232047

Fig 3. 129 Dnd1+/Δ male mice progressively lost fertility because of sperm count decrease and sperm malfunction.

Fig 3

(A–D) Litter size analysis of Dnd1+/Δ male mice of the BL6, MCH, and 129 strains and wild-type male mice of the 129 strain. A 10-week-old male mouse was crossed with three wild-type female MCH mice until the male mouse reached 24 weeks of age. Three male mice (Mouse 1, Mouse 2, and Mouse 3) were analyzed per strain or genotype. (E–G) Comparison of testis weight per body weight ratios between 4-week-old and 12-week-old wild-type and Dnd1+/Δ mice from the BL6, MCH, and 129 strains. Error bars represent mean ± SD; three mice were analyzed per genotype and strain. *P < 0.01, **P < 0.005 (Student’s t-test). (H–J) Sperm count analysis of 12-week-old wild-type and Dnd1+/Δ male mice of the BL6, MCH, and 129 strains. Error bars represent mean ± SD; three mice were analyzed per genotype and strain. *P < 0.01, **P < 0.005, ***P < 0.001 (Student’s t-test). (K) IVF analysis using sperm from more than 12-week-old wild-type and Dnd1+/Δ male mice of the 129 strain. Error bars represent mean ± SD; three mice were analyzed per genotype. ***P < 0.001 (Student’s t-test).