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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Am J Transplant. 2018 Sep 17;19(3):724–736. doi: 10.1111/ajt.15067

FIGURE 5.

FIGURE 5

Posttransplant DSA and antibody-mediated rejection was significantly alleviated with costimulation blockade and proteasome inhibitor based desensitization. (A) C4d deposition in renal biopsies taken at the time of rejection after transplant. Animals treated with desensitization showed lower C4d score. (B) Antibody-mediated rejection score (Banff g + ptc + C4d) in end-of-study histology samples. Animals treated with desensitization treatment showed lower AMR scores than control animals. (C) Comparison of posttransplant course of DSA (normalized to prerenal transplant value = 1). Animals with desensitization did not show increased serum DSA. (D) Pearson’s correlation coefficients were calculated between DSA (at sacrifice) by T cell and B cell flow crossmatch to the calculated AMR score. Circulating DSA at sacrifice from both control and desensitized animals showed strong correlation (r = 0.68 and P = .02 for T cell FXM; r = 0.59 and P = .058 for B cell FXM) to the AMR score. (E) Representative immunofluorescence image of GC staining with CD3 (blue), CD20 (red), and Ki67 (green) in mesenteric and peripheral lymph node sections. Lymph nodes were collected at sacrifice (peripheral LNs)