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. 2016 Nov 23;22(2):234–248. doi: 10.1016/j.drudis.2016.10.017

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© 2016 Elsevier Ltd. All rights reserved.

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Transient biosynthesis of AMPs using the Magnifection platform. (a) Assembled viral vector cassettes harboring the coding sequence of an AMP is introduced in cells of A. tumefaciens. After growth and selection of transgenic bacteria (b), leaves of N. benthamiana are agroinfiltrated using a syringe or vacuum (c) and the kinetics of transgene transient expression reaches its peak between 4 and 7 days after transfection (d). After harvesting the plant biomass (e) and peptide extraction using appropriate buffers (f), the AMPs are purified and evaluated in bio-assays (g).