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. 2020 Jan 31;238:119828. doi: 10.1016/j.biomaterials.2020.119828

Fig. 4.

Fig. 4

Effect of Mg and VC on prevention of SAON and suppress the apoptosis of osteocytes. (A) Representative H&E staining images showed ON, indicated by the empty lacunae (black arrow) in trabecular bone or pyknotic nucleus of osteocytes (blue arrow). (B) The incidence of SAON in each group. All the SAON model rats developed ON at 2 weeks after induction. The incidence of SAON decreased at 6 weeks after induction, suggested progressive repair after week 2. VC further significantly decreased the incidence SAON at 6 weeks after induction. (*p < 0.05, **p < 0.01; Chi-square test, n = 8; osteonecrosis+: with osteonecrosis detected by histology; Osteonecrosis-: without osteonecrosis detected by histology) (C) Representative TUNEL staining images showed apoptosis of osteocytes. (D) Apoptosis rate indicated by percentage of TUNEL-positive osteocytes. (*p < 0.05, **p < 0.01; one-way ANOVA; aa: p < 0.01, for Mg factor; bb: p < 0.01, for VC factor, two-way ANOVA; n = 8).