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. 2020 Jan 31;238:119828. doi: 10.1016/j.biomaterials.2020.119828

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Fig. 6 — Ex vivo cell culture. (A) The Bone marrow MSCs of rats at 2 weeks after SAON induction from every group were cultured ex vivo, and then differentiated in osteogenic induction medium. Representative images of Alizarin red staining and qPCR of ALP and COL I A1 showed the potential of osteogenic differentiation of MSCs in each group. (B) The bone marrow macrophages of rats 2 weeks after SAON induction from every group were cultured ex vivo and differentiated in osteoclastic induction medium. Quantitative analysis of TRAP staining images about osteoclast number and size and qPCR of Cathepsin K expression showed the osteoclast differentiation potential of BMMs in each group (*p < 0.05, **p < 0.01, vs. SAON; #: p < 0.05, vs. VC, n = 4).