Fig 7.

Circulating CXCL9 and CXCL10 levels correlate with disease parameters in the model of TLR9-induced sHLH. Mice were injected with 50 μg of CpG-ODN at days 0, 2, 4, 7, and 9. Serum levels of CXCL9, CXCL10, and IFNγ were quantified in a multiplex assay using the Luminex technology. Blood parameters including platelet and lymphocyte counts were measured using a hemavet analyzer, and serum ferritin was measured by ELISA. Correlations were performed between CXCL9 and disease parameters, CXCL10 and disease parameters, and IFNγ and disease parameters at different time point as HLH develops (depicted in Fig 1). Statistics were performed and P values were obtained using the Spearman test. *P < 0.05, **P < 0.01, and ***P < 0.001. The line of best fit was generated using the nonlinear regression analysis using GrapPad Prism software. TLR9, Toll-like receptor 9; sHLH, secondary hemophagocytic lymphohistiocytosis; IFNγ, interferon γ.