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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Cancer Lett. 2018 Jul 21;434:144–151. doi: 10.1016/j.canlet.2018.07.024

Figure 1. Inhibition of Notch1 inhibits melanoma growth and affects the tumor microenvironment.

Figure 1.

A) Expression of mouse specific shNotch1 in B16F10 (left) and Yumm1.1 (right). shN1 #1 was used for the in vivo growth. B) Tumor growth of B16F10 and Yumm1.1 mouse melanoma cells. C) % MDSCs (CD11b+; Ly6Chi; ly6G− = monocytic; and CD11b+; Ly6Clo; Ly6G+ = polymorphonuclear) in B16F10 and Yumm1.1 tumors expressing shGFP or shNotch1 (n=20). D) %MDSCs in spleen of mice bearing B16F10 and Yumm1.1 tumors expressing shGFP or shNotch1 (n=20). E) Arginase-1, IL10 and TGFβ expression detected by quantitative PCR in B16F10 and Yumm1.1 tumor lysates, normalized to β-actin.