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. Author manuscript; available in PMC: 2020 Apr 27.
Published in final edited form as: Cancer Lett. 2018 Jul 21;434:144–151. doi: 10.1016/j.canlet.2018.07.024

Figure 5. Notch1 controls the expression of SNAP23.

Figure 5.

A) mRNA expression levels of several components of the SNAREs (soluble N-ethylmaleimide–sensitive factor attachment protein [SNAP] receptors) family of membrane fusion proteins in WM266-4 cells depleted of Notch1. B) mRNA expression of SNAP23 in cells depleted of all Notch receptors, normalized to β-actin. C) SNAP23 protein expression in several human melanoma cell lines expressing shGFP or shNotch1. D) SNAP23 shRNA knock down levels. E) ELISAs for IL-8, IL-6 and CCL5. Conditioned media from WM266-4 cells depleted of SNAP23 was used. shSNAP23/2 was used in the experiment. Treatment was done as in fig. 3C and chemokine content reported as pg/ug total protein in lysates as a relative measure of cell density. *,**p<0.01. Values are the mean of three independent experiments run in triplicate.