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. 2016 Jan 13;40(4):494–503. doi: 10.1016/j.clinre.2015.11.003

Figure 3.

Figure 3

ALDH activity and CD13 expression of HSC-58 cells. A. HSC-58 cells were analyzed for ALDH activity as in the legend of Fig. 2, and were also analyzed by FACS for CD13 expression. Horizontal axes denote the expression intensity of ALDH activity, the vertical axes denote CD13 expression (upper panels). The shaded histogram indicates CD13 expressing cells with low ALDH activity. The open histogram (thick line) indicates CD13 expressing cells with high ALDH activity. B, C. Drug sensitivity test of HSC-58 cells sorted into CD13-high (open circles) and CD13-low populations (closed circles). HSC-58 cells were separated by AutoMACS and sorted cells are exposed to the indicated concentrations of cisplatin (B) or bestatin (C) for 5 days following which viable cells were counted by fluorescence analysis of reduction of the AlamarBlue® reagent. Data are analyzed and represented by GraphPad Prism ver. 4.0 software.