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. 2016 Jan 13;40(4):494–503. doi: 10.1016/j.clinre.2015.11.003

Figure 4.

Figure 4

Targeted delivery of an immunotoxin into HSC-58 cells. A. Expression of CD13 and EpCAM on HSC-58 cells as analyzed by flow cytometry. B. Cytotoxicity of a saporin-conjugated second antibody targeted against anti-CD13, anti-EpCAM antibody or control IgG bound to HSC-58 cells was assayed using fluorescence analysis of reduction of the dye AlamarBlue®. The horizontal axis denotes the concentration of the saporin immunotoxin. The vertical axis indicates cell survival. C. Phase-contrast images of representative cells treated with isotype control IgG (negative control; top right), isotype control IgG plus saporin-conjugated second antibody (bottom left) and anti-CD13 IgG plus saporin-conjugated second antibody (bottom right) for 5 days.