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Position of labeling sites on the P450cam structure (PDB ID 3l63) to probe F-helix movements. One 4MT label is attached to a reference position at S48C, and the other 4MT label is attached to the outwardly pointing Y179C in the middle of the F helix. The CA and CB atoms of S48C and Y179C are shown as yellow spheres, and the heme and ion-sulfur cluster are shown as red sticks. Closed P450cam is shown in cyan, the F/G helices in the open form are shown in pink, and Pdx is in blue.
