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. 2020 Apr 1;31(8):793–802. doi: 10.1091/mbc.E19-12-0697

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© 2020 Zhao et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 4: — ORP1S partially localizes on the PM. (A) Colocalization of ORP1S-GFP and mCherry-D4H on the PM of an ORP1-null cell (bar, 5 μm). (B) ORP1-null cells expressing ORP1S-Flag were cultured in 10% FCS and immunostained with an anti-Flag monoclonal. (C, D) ORP1-null cells expressing ORP1S-GFP were cultured in delipidated medium without or with LDL (50 μg/ml) for 16 h (bar, 20 μm). All images are confocal sections (1 μm), and magnified regions are boxed.