FIGURE 7:

Reduced ABCA1-mediated cholesterol efflux in ORP1-null cells. (A) Cells received no addition (NA, solvent control), 1.0 µM T0901317, and 10 nM RA or 1 μM 22OH and 10 nM RA for 16 h. Cell lysates were immunoblotted for ABCA1 and OSBP. (B) Expression of ABCA1 was quantified relative to OSBP load control. Results are the mean and SD of six experiments (unpaired t test, *p < 0.05, **p < 0.001 compared with similarly treated HeLa cells). (C) The percentage of efflux of [³H]cholesterol to apoAI was measured as described in Materials and Methods. Results are the mean and SD of five experiments (unpaired t test, *p < 0.05, **p < 0.005 compared with similarly treated HeLa cells).