FIGURE 2:
Overexpression of wdp reduces the Hh-signaling-active domain. (A–C) A control adult wing (A) and adult wings expressing UAS-wdp with BxMS1096-GAL4 (B, C). Longitudinal wing veins 3 and 4 are marked (L3 and L4, respectively). (D, E) Quantification of the whole adult wing area (D) and the ratio of the area of the L3–L4 domain relative to the whole wing (E) for BxMS1096-GAL4/+ (control), BxMS1096-GAL4>wdp (wdp), and BxMS1096>wdpΔGAG (wdpΔGAG); n.s., not significant; ***p < 0.001. The Wilcoxon rank sum test was used to test the statistical significance (n = 12 in control; n = 14 in wdp; n = 18 in wdpΔGAG). (F–K) Control wing discs (ap>GFP) (F, H, and J) and wing discs overexpressing wdp with ap-GAL4 (ap>GFP+wdp) (G, I, and K) were immunostained for the expression of Ptc, Ci (F and G), dpp-lacZ (H and I), En, and hh-lacZ (J and K). Nuclei were stained with DAPI. The expression domains of Ptc, Ci, and dpp-lacZ were reduced by wdp overexpression in the dorsal compartment compared with those in the ventral compartment. En expression induced by high-level Hh signaling in the anterior compartment was also diminished by wdp overexpression. Note that the hh-lacZ expression was not affected by wdp overexpression. Anterior to the left; dorsal to the top. Scale bars: 50 µm. (L, M) Signal intensity plots of the Ptc expression in the dorsal (red) and ventral (blue) compartments in ap>GFP (L) and ap>GFP+wdp (M). Solid lines indicate the average intensity of Ptc staining, and shaded areas show the standard error of the mean (n = 21 in L; n = 17 in M). The difference between the distance (x value) of the Ptc mean intensity maximum and the x value of two-thirds of the maximum on the anterior (A) side was measured for each peak. These values were used to compare the shape of the Ptc expression patterns between the dorsal and ventral compartments. P values were calculated using the Wilcoxon rank sum test.