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. 2020 Apr 1;31(8):813–824. doi: 10.1091/mbc.E19-06-0327

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FIGURE 5: — wdp RNAi knockdown leads to increased Hh signaling. (A) A wing disc expressing UAS-wdp^RNAi (TRiP.HMC06302) with ap-GAL4 was immunostained for Ptc and Ci. (B, C) Signal intensity plots of the Ptc expression in the dorsal compartment (red) and ventral compartment (blue) in wing discs expressing UAS-Flp (B) and UAS-wdp^RNAi (C). Solid lines indicate the average intensity of Ptc staining and shaded areas show the standard error of the mean (n = 10). P values were calculated using the Wilcoxon rank sum test via the method described in Figure 2. (D, E) A control wing disc (D) and a wing disc expressing UAS-wdp^RNAi (TRiP.HMC06302) with ap-GAL4 (E) were immunostained for dpp-lacZ. Nuclei were stained with DAPI. Scale bars: 50 µm.