FIGURE 5:

Partial ROCK inhibition accelerates collective cell migration. Dose-dependent effect of Y-27632 on collective MDCK cell migration velocity. (A) DIC images of migrating cell protrusion in the presence of the indicated Y-27632 concentration at the start (0 min) and end (300 min) of the time-lapse observation. Trajectories of the individual cells were overlaid. Cells were tracked every 5 min for 5 h. Scale bar = 100 μm. (B) Velocities of the collective cell migration are shown as average velocities of the tracked cells in each condition. Data are means ± SD of three independent experiments (29–32 cells/experiment). *, P < 0.05 (one-way ANOVA followed by Dunnett’s test).