FIGURE 3:

Blocking p150 dynactin leads to fewer processive events and more diffusive events following release from Apo-lock. (A) Diagram of the Apo-lock experiment. DDB complexes bind to immobilized microtubules in absence of ATP, and ATP buffer is flushed into the system to initiate motility. (B) Kymograph of DDB motility 5 min after flowing in ATP buffer for control (left) and in the presence of Ab_p150 (right). Processive (P), diffusive (D), and stuck (S) events are noted. (C) Average fraction of processive, diffusive, and stuck traces across n = 10 kymographs for control (black) and n = 10 Ab_p150 group (blue). Error bars are SEM; ** denotes p < 0.01; * denotes p < 0.05 (two-tailed t test); n.s., not significantly different.