FIGURE 3:

Loss of ERK7 blocks parasite invasion and egress. (A) ERK7^AID parasites were incubated as indicated with IAA and lysates were probed with anti-FLAG and anti–β-tubulin as a loading control. (B) Quantification of three replicate plaque assays with parental, ERK7^AID, and ERK7^AID(wt-comp) strains +/− IAA. (C) Phase image of ERK7^AID parasites grown 24 h +/− IAA. (D) Parasites as in C were grown 18 h more until vacuoles began to rupture. (E) Quantification of egress from n = 3 replicates grown as indicated in IAA. (F) Quantification of attached and invaded parasites from ERK7^AID and ERK7^AID(wt-comp) strains grown +/− IAA. (G) Quantification of Gaussia luciferase activity secreted by the indicated strains grown +/− IAA. (H) ERK7^AID and ERK7^AID/IAA expressing an actin chromobody (magenta) and secreting Venus into the PV (green) were imaged at the indicated times after ionophore treatment. Scale bars: 10 μm. (I) Quantification of PV Venus signal of parasites as in H relative to the first frame (n = 93 −IAA; 81 +IAA). Error bars are SD.