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Influence of Retromer on endosomal recycling. (A) WT and mutant cells expressing GFP-Snc1 were imaged at 1000×. Images shown are single planes chosen from a z-stack series. (B) Quantification of images captured in A (n = 50). (C) Measurement of FM4-64 postendocytic recycling in WT and mutant cells. Fluorescence intensity was normalized to the initial value for each strain. Data represent three independent experiments.
