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. 2020 Apr 1;9:e54532. doi: 10.7554/eLife.54532

Figure 5. Family specific conserved features in the HV regions correlate with acceptor recognition and specificity.

Figure 5.

Conserved residues in A) HV2 of the DPM1 sequences in the GT2-DP subfamily coordinate the phosphate group of the acceptor. (B) HV1 of GT16 MGAT1 provide acceptor specificity. (C) HV2 and HV3 of EXTL GT64 family (C-terminal GT domain of the multidomain sequences) coordinate the acceptor. Left: Alignments highlighting the constrained residues are shown for each family. The family specific conserved residues are shown using black dots above the alignment. Red bars above these dots indicate the significance of conservation (Higher bar corresponds to more significantly conserved position). Right: Representative pdb structures are shown for each family (GT2-DP:5mm1, GT16:5vcs, GT64:1on8); Donor substrates are colored brown. Acceptors are colored purple. HVs are highlighted in orange. The position of the conserved DxD and xED motif for each structure is shown as cyan and magenta circles respectively.