Skip to main content
. 2020 Mar 23;9:e52676. doi: 10.7554/eLife.52676

Figure 2. Effects of pdm3 knockdown on sleep architecture.

(A) Day sleep average bout length and (B) day sleep average bout number in pdm3 RNAi and controls at day 1 (blue) versus day 4–5 (orange). (C) Night bout length and (D) night average bout number in pdm3 RNAi and controls (n = 97, 106, 119, 95, 140, 145 left to right in A-D). (E) Total sleep time at day 1, pdm3 RNAi versus controls (n = 97, 119, 140 left to right). (F) Minutes of sleep lost in Elav-GAL4 >UAS pdm3 RNAi at day one compared to each genetic control. (G) Total sleep time at day 4–5, pdm3 RNAi versus controls (n = 106, 95, 145 left to right). (H) Minutes of sleep lost in Elav-GAL4 >UAS pdm3 RNAi at day 4–5 compared to each genetic control. ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05; multiple Student’s t tests with Holm-Sidak correction, alpha = 0.05 (A–D); ANOVA with Tukey’s test (E–H).

Figure 2.

Figure 2—figure supplement 1. Pdm3 knockdown disrupts behavioral rhythms but leaves molecular clock intact.

Figure 2—figure supplement 1.

(A) Quantification of sleep redistribution as ratio of day/night sleep in mature flies (n = 106, 95, 145 left to right). (B) FFT amplitude of free-running rhythms in constant darkness (mature flies) (n = 29, 25, 28 left to right). (C) Classification of rhythm strength based on FFT value. (D) Representative actograms for each genotype in constant darkness. (E) Intensity of PER protein levels in sLNv core clock cells across the circadian day in pdm3 knockdown and controls (n = 7–9 brains per condition).
Figure 2—figure supplement 2. Comparison of sleep duration within age groups with pdm3 knockdown.

Figure 2—figure supplement 2.

Day (A) and night (B) sleep duration at day 1, pdm3 RNAi versus controls (n = 97, 119, 140 left to right). Day (C) and night (D) sleep duration at day 4–5, pdm3 RNAi versus controls (n = 106, 95, 145 left to right) ****p<0.0001, ***p<0.001, **p<0.01, *p<0.05; ANOVA with Tukey’s test (A–D).
Figure 2—figure supplement 3. Maturation of courtship behaviors are unaffected by pdm3 knockdown.

Figure 2—figure supplement 3.

Courtship Index (time spent engaged in courtship behavior/total assay time) at day 1 versus day 4–5 in controls and pdm3 RNAi (darker bars, right) (n = 28, 27, 21, 28, 23, 28 left to right). ****p<0.0001; multiple Student’s t tests with Holm-Sidak correction, alpha = 0.05.