Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene Drosophila melanogaster | pdm3 | FBgn0261588 | ||
| Gene Drosophila melanogaster | Msp300 | FBgn0261836 | ||
| Genetic reagent (D. melanogaster) | hs-hid; Elav-GAL4; UAS Dcr2 | Dragana Rogulja | ||
| Genetic reagent (D. melanogaster) | UAS-pdm3 RNAi | BloomingtonDrosophilaStock Center | BSC #53887, TRiP HMJ21205 | |
| Genetic reagent (D. melanogaster) | UAS-pdm3 RNAi | ViennaDrosophilaResource Center | VDRC #30538, Construct ID 4312 | |
| Genetic reagent (D. melanogaster) | ElavC155-GAL4 | Amita Sehgal | ||
| Genetic reagent (D. melanogaster) | UAS-mCD8-GFP | BloomingtonDrosophilaStock Center | ||
| Genetic reagent (D. melanogaster) | UAS-pdm3-short | John Carlson | ||
| Genetic reagent (D. melanogaster) | tubGAL80-ts | BloomingtonDrosophilaStock Center | BSC #7017 | |
| Genetic reagent (D. melanogaster) | R23E10-LexA | BloomingtonDrosophilaStock Center | BSC #52693 | |
| Genetic reagent (D. melanogaster) | TH-LexA | Ronald Davis | ||
| Genetic reagent (D. melanogaster) | LexAOp-GFP | BloomingtonDrosophilaStock Center | BSC #32203 | |
| Genetic reagent (D. melanogaster) | LexAOp-Brp-short-mCherry | Takashi Suzuki | ||
| Genetic reagent (D. melanogaster) | 23E10-GAL4 | BloomingtonDrosophilaStock Center | BSC #49032 | |
| Genetic reagent (D. melanogaster) | UAS-CaLexA | J. Wang | ||
| Genetic reagent (D. melanogaster) | ElavC155-QF2 | BloomingtonDrosophilaStock Center | BSC #66466 | |
| Genetic reagent (D. melanogaster) | QUAS-pdm3 RNAi | This study | Figure 4—figure supplement 2; available upon request | |
| Genetic reagent (D. melanogaster) | Dop1R1[attp] | Krystyna Keleman | ||
| Genetic reagent (D. melanogaster) | UAS-Dcr2 | BloomingtonDrosophilaStock Center | BSC #24651 | |
| Genetic reagent (D. melanogaster) | UAS-Msp300 RNAi | ViennaDrosophilaResource Center | VDRC #105694 | |
| Genetic reagent (D. melanogaster) | UAS-Msp300 RNAi | ViennaDrosophilaResource Center | VDRC #109023 | |
| Genetic reagent (D. melanogaster) | UAS-Msp300 RNAi | ViennaDrosophilaResource Center | VDRC #107183 | |
| Antibody | anti-PDM3 (Guinea pig polyclonal) | Cheng-Ting Chien | 1:500 | |
| Antibody | anti-GFP (Mouse monoclonal) | Sigma | Cat# G6539-200UL | 1:500 |
| Antibody | anti-GFP (Rabbit polyclonal) | Fisher | Cat# A11122 | 1:500 |
| Antibody | anti-PER (Guinea pig polyclonal) | Amita Sehgal | 1:1000 | |
| Antibody | anti-PDF C7 (Mouse monoclonal) | Developmental Studies Hybridoma Bank | 1:500 | |
| Antibody | anti-nc82 (Brp) (Mouse monoclonal) | Developmental Studies Hybridoma Bank | 1:100 | |
| Antibody | Alexa Fluor 488 Donkey anti-Rabbit | Thermo Fisher | 1:1000 | |
| Antibody | Alexa Fluor 555 Donkey anti-GP | Thermo Fisher | 1:1000 | |
| Antibody | Alexa Fluor 488 Donkey anti-Mouse | Thermo Fisher | 1:1000 | |
| Antibody | Alexa Fluor 647 Donkey anti-Mouse | Thermo Fisher | 1:1000 | |
| Recombinant DNA reagent | QUAS-WALIUM20 (vector) | Jonathan Zirin, Fly Transgenic RNAi Project | ||
| Sequence-based reagent | HMJ21205 pdm3 RNAi hairpin | Synthesized in this study, sequence from TRiP database | ||
| Restriction enzyme | NheI | New England Biolabs | Cat. #: R3131S | |
| Restriction enzyme | EcoRI | New England Biolabs | Cat # R3101L | |
| Commercial assay or kit | Plasmid Midi Kit | Qiagen | Cat # 12143 | |
| Software, algorithm | Prism 8 | Prism |