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. 2020 Apr 21;12:2653–2662. doi: 10.2147/CMAR.S239418

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© 2020 Du et al.

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Upregulation of UPK1A-AS1 inhibits ESCC cell proliferation, migration, and invasion. EC109 and KYSE30 cells were transfected with UPK1A-AS1 or vector. (A) qRT-PCR validation of UPK1A-AS1 upregulation in EC109 and KYSE30 cells following transfection with UPK1A-AS1 overexpressing plasmid. (B and C) Cell viability was evaluated at 48 h post-transfection using CCK-8 assay. (D and E) Plate colony formation assay and soft agar colony formation assay of EC109 and KYSE30 cells transfected with UPK1A-AS1 or vector. (F and G) Scratch-healing and transwell invasion assays were performed to assess the migration and invasion of EC109 and KYSE30 cells at 48 h post-transfection, respectively. Scale bar: 200 μM. **P < 0.01, ***P < 0.001.