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. 2020 Apr 21;12:2653–2662. doi: 10.2147/CMAR.S239418

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© 2020 Du et al.

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UPK1A-AS1 regulates ESCC cell proliferation, migration, and invasion partially by sponging miR-1248. (A and B) qRT-PCR analysis of miR-1248 expression in EC109 and KYSE30 cells following transfection with UPK1A-AS1 and miR-1248 mimic. (C and D) Results of CCK-8 assay in EC109 and KYSE30 cells transfected with UPK1A-AS1 overexpressing plasmids alone or co-transfected with UPK1A-AS1 overexpressing plasmids and miR-1248 mimic. (E and F) Results of plate colony formation assay and soft agar colony formation assay in EC109 and KYSE30 cells transfected with UPK1A-AS1 alone or with miR-1248 mimic. (G and H) Results of scratch-healing assay in EC109 and KYSE30 cells transfected with UPK1A-AS1 alone or with miR-1248 mimic. (F) Results of transwell invasion assay in EC109 and KYSE30 cells transfected with UPK1A-AS1 alone or with miR-1248 mimic. *P < 0.05, **P < 0.01, ***P < 0.001.