Fig. 2.

Viability of breast cancer cells growing in tumor spheres representing hypo-vascularized BCLM in 3D co-culture with M1 and M2 polarized macrophages. M1 macrophages were CRISPR-RICTOR-Liposomes treated and differentiated in the presence of IFN-gamma/LPS, while M2 macrophages were polarized in vitro in the presence of IL-4/M-CSF. The viability is shown as a function of varying ratios of M1:M2 macrophages under conditions of no treatment or exposure to MSV-nab-PTX for a 48 h and b 72 h. The varying ratios mimic the varying strength of a hypothetical polarization regimen that shifts this ratio. Mean ± SD, biological replicates n = 5, *p < 0.05, **p < 0.01 vs control