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. 2020 Mar 10;295(17):5614–5625. doi: 10.1074/jbc.RA120.012588

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© 2020 Maddur et al.

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Figure 3. — Characterization of SC(1–246)-BODIPY and its complex with ProT^QQQ. A, SDS-PAGE showing the purity of the labeled probe, SC(1–246)-BODIPY. 5 μg of the labeled probe was separated under reduced (lane 2) and nonreduced (lane 4) conditions. Lane 1, protein standards; lane 3, blank. B, native gel electrophoresis was used to show the SC(1–246)-BODIPY·ProT^QQQ complex formation. ProT^QQQ (2.5 μm, lane 1) was incubated with SC(1–246)-BODIPY (0-fold (lane 2), 0.5-fold (lane 3), 1.0-fold (lane 4), and 1.5-fold (lane 5) excess of ProT^QQQ) for 15–30 min at 25 °C, and the complex was separated on native PAGE at 4 °C.