Figure 11.

PP2A/B′δ reduction identifies consensus dephosphorylation motifs in the global phosphoproteome. Phosphopeptides depleted ≥1.5-fold by PP2A/B′δ RE and OE were subjected to motif analysis. A, motif scores (means ± S.E.) of the 45 (RE) and 6 motifs (OE) identified by pLogo (see supporting Excel File S2 for a list of motifs). The p value is based on Student's t test. B and C, motif logos for phospho-Ser- (top) and phospho-Thr–containing peptides (bottom) depleted by PP2A/B′δ RE (B) and OE (C). Pink down arrows indicate positions with significantly enriched residues, which are identified by heights exceeding the significance threshold (red line at +3.75).